The Control Room first appears on 10F during the first time Agent 8 and Pearl climb the Spire with Acht's help in order to find and save Marina. In the center, Marina is being mind controlled to stop anybody who enters the spire as Marina Agitando.
After defeating and saving Marina, Order appears and throws the three of them out the spire. From then on, the Control Room moves to the 30th floor of the spire.
On a normal climb, the Control Room appears on 30F instead and is guarded by Order. After being confronted by Agent 8, Pearl, and Marina, Order transforms into Overlorder. The player must first go through three phases of the boss fight, after which a fourth phase unique to the first time defeating Overlorder will take place. Defeating Overlorder transforms it into Smollusk. The credits plays afterwards.
The fourth phase can be re-experienced by selecting "Remember Epic, Vibrant Battle" from the glass case on viewer's right upon entering the foyer.
The Control Room is still on 30F, but it is now guarded by Smollusk. It still transforms into Overlorder and the player must go through three phases of the boss fight. The fourth phase does not take place, however, as Overlorder returns into Smollusk form upon being defeated in the third phase.
Quotes
Translation needed
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Marina Agitando
English
While riding the cage on 10F:
Acht
Is that... no way.
Before the fight with Marina Agitando:
Pearl
Marina!
Pearl
Hey, 'Rina! Yo, can she even hear me?!
Pearl
Guess not. She's all tangled up in that weird machine!
Marina
Intruders confirmed. Commence Grayscaling.
Pearl
Sit tight! We're gonna get you outta that thing!
Pearl
Ready to rock, Eight?!
During the fight with Marina Agitando:
Acht
That's a powerful barrier between us and Marina.
Pearl
Well, now we know what to take out first!
Marina
Detecting resistance to Grayscaling.
Pearl
Is this a portal? It's kinda funny looking.
Pearl
Scratch one portal!
Acht
Hey, I think those portals are powering the barrier.
Cancel that snooze button! Hit me with the beat, DJ!
Marina
Coming at you, Pearl!
Overlorder
Of what use is this resistance.
Overlorder
Happiness is stasis. Unchanging. Forever.
Pearl
You kidding? We're still on tour!
Pearl
The show don't STOP!
Pearl
Keep your boring gigs for boring people. We're out here LIVING, loser!
Pearl
Right, gang? Crank it up!
Marina
Mic check!
Pearl
Boo-YAAAAAAHHHHHH!
The One Slight Catch
Before defeating Smollusk:
Pearl
Whoa! Hey there, Smollusk! Don't mind us, buddy. We'll be out of your way in a sec!
Pearl
So like...anytime you wanna step aside is good.
Smollusk
You got some nerve showing up in MY pwayroom and running your mouth! HAWWUMPH!
Pearl
Yo, does it seem kinda...different from the way Order acted?
Marina
Kind of. Your wail might have sheared off some core data in our last battle.
Smollusk
You wuined my dweam! And not just mine-evewyone else's too! You big, stinking, wotten MEANIES!
Smollusk
This time, I'm gonna chop you into teeny-weeny octo bits!
After defeating Smollusk:
Smollusk
Nooo! You cheaters beat me again! No fair, no fair, NO FAIR!
Marina
Nice going, Eight! Another palette reconfigured.
Smollusk
FINE, THEN! But this isn't over! I'm gonna keep twying for everyone's dreams!
Trivia
In the fourth phase of fighting Overlorder, once all portals are destroyed and all chips are recovered, opening the Palette menu and interacting with each chip in boustrophedon order (left-to-right for odd rows and right-to-left for even rows) sounds out the chorus of Ebb & Flow.
Eight's Palette is the only Palette that the fourth phase cannot be replayed with. It is reverted to Agent 4's Palette upon attempting to do so.