Opening is a short guitar sequence with an added drums and a reverb effect, and plays at the beginning of every multiplayer match in Splatoon, as the camera pans out across the stage, before being cut off by the players turning into humanoid form on the spawn point. Normally the full track does not play, but under lag, it may sometimes play the full track, and after it finishes, the silence continues as the camera pan repeats.
Remixes and covers
Opening (Squid Sisters)
During Splatfests in Splatoon, all the regular music was replaced with Squid Sisters songs. Therefore, instead of the standard Squid Squad version, a cover was used by the Squid Sisters, despite a lack of vocals. It is now a purely electronic production. It was also used at the beginning of Live Performances, and recordings of the intros from Shiokaraibu at Tokaigi 2016 and Shiokaraibu at Chokaigi 2016 can be found in Splatoon Live in Makuhari.
Opening (Wet Floor)
No new Squid Squad songs appear in Splatoon 2 matches (later revealed to be due to the group's disbandment), with Wet Floor taking their place. Although their catalog consists mostly of all-new compositions, they also recorded a new version of the Opening jingle, sounding very similar to the original, yet with additional electronic influences.
Opening (Off the Hook)
Like what the Squid Sisters did in Splatoon, Off the Hook recorded a new version for use in Splatfests in Splatoon 2, with heavy electronic production. Once again, it contains no vocals from Off the Hook. It is also used at the beginning of live performances, and recordings of the intros from Haicalive at Tokaigi 2018 can be found in Octotune.
Last Battle Opening
A minor key version of the Off the Hook version of the opening is heard at the beginning of Turf War in the Octo Expansion. This version is extended as well, as it plays when Marina is explaining the instructions to Agent 8. It was also used in the Chaos vs. Order Splatfest, except with it ending a little bit sooner, replacing the standard Off the Hook version.